Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods

1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments

Douglas W. Houston

2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

Thao Nguyen, Timothy J. Mitchison, and Martin W?hr

3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos

Michael D. Sheets

4. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

Alexander A. Tokmakov and Ken-Ichi Sato

5. Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis

Ken-ichi Sato and Alexander A. Tokmakov

6. Embryological and Genetic Manipulation of Chick Development

Laura S. Gammill, Bridget Jacques-Fricke, and Julaine Roffers-Agarwal

7. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

Ana Paula Azambuja and Marcos Simoes-Costa

8. Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens

Triveni Menon and Sreelaja Nair

9. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)

Karoline Holler and Jan Philipp Junker

10. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos

Clare E. Buckley

11. Ex Utero Culture and Imaging of Mouse Embryos

Sonja Nowotschin, Vidur Garg, Anna Piliszek, and Anna-Katerina Hadjantonakis

12. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn, Miriam Holzman, and Jennifer H. Mansfield

13. Reptile Embryology and Regeneration

Matthew K. Vickaryous and Emily A.B. Gilbert

14. Manipulation of Developmental Function in Turtles with Notes on Alligators

Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, and Judith A. Cebra-Thomas

15. Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes

Amanda Butler, Dawn Owens, Mary Lou King, and Tristan Aguero

16. Visualizing the Balbiani Body in Zebrafish Oocytes

KathyAnn L. Lee and Florence L. Marlow

17. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis

Allison Jamieson-Lucy and Mary C. Mullins

18. Generation of

• Format: Hardcover
• Language: English
• ISBN: 149399008X
• ISBN13: 9781493990085
• Release Date: February 2019
• Publisher: Humana
• Length: 434 Pages
• Weight: 2.18 lbs.
• Dimensions: 1.0" x 7.0" x 10.0"