Practical Methods for Biocatalysis and Biotransformations

By John Whittall

Biocatalysts are increasingly used by chemists engaged in finechemical synthesis within both industry and academia. Today, thereexists a huge choice of high-tech enzymes and whole cellbiocatalysts, which add enormously to the repertoire of syntheticpossibilities.

Practical Methods for Biocatalysis and Biotransformationsis a "how-to" guide focussing on commercially availableenzymes and strains of microorganisms that are readily obtainedfrom culture collections. The source of starting materials andreagents, hints, tips and safety advice (where appropriate) aregiven to ensure, as far as possible, that the procedures arereproducible. Comparisons to alternative methodology are given andrelevant references to the primary literature are cited. Contentsinclude:

Introduction to the application of biocatalysts in the pharmaindustryintroduction to molecular biological techniques for biocatalystdevelopmentLipase and protease methods including enzyme supports anddynamic kinetic resolutionsNitrile hydrolases and hydratasesAlcohol dehydrogenases and co-factor recyclingEpoxide hydrolasesAmino acid synthesisWhole cell processesOther biocatalysts, including monoamineoxidases, aminotransterases, and lyases

Practical Methods for Biocatalysis and Biotransformationsis an essential text offering validated biocatalyst methods forresearchers and postgraduate students in industry and academia.

• Format: Hardcover
• Language: English
• ISBN: 0470519274
• ISBN13: 9780470519271
• Release Date: December 2009
• Publisher: Wiley
• Length: 432 Pages
• Weight: 1.95 lbs.
• Dimensions: 1.1" x 6.8" x 9.8"