Plant Micrornas: Methods and Protocols

1. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants

Alma Armenta-Medina and C. Stewart Gillmor

2. The Plant MicroRNA Pathway: The Production and Action Stages

Joseph L. Pegler, Christopher P. Grof, and Andrew L. Eamens

3. Plant miRNA Conservation and Evolution

Lydia Gramzow and G?nter Thei en

4. Bioinformatic Analysis of Small RNA Sequencing Libraries

Ricardo A. Ch?vez Montes, Fabiola Jaimes Miranda, and Stefan de Folter

5. An Overview of miRNA and miRNA Target Analysis Tools

Anna Lukasik and Piotr Zielenkiewicz

6. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences

Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, and Chuang Ma

7. Prediction of Plant miRNA Targets

Priyanka Pandey, Prashant K. Srivastava, and Shree P. Pandey

8. Isolation and Detection Methods of Plant miRNAs

Pedro Fernando Vera Hern?ndez, Stefan de Folter, and Flor de F?tima Rosas C?rdenas

9. Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants

Carlos De la Rosa and Jos? Luis Reyes

10. miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses

Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak, and Zofia Szweykowska-Kulinska

11. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization

Marcelino Mart?nez N??ez, Stefan de Folter, and Flor de F?tima Rosas C?rdenas

12. In Situ Localization of Small RNAs in Plants

Cristina F. Marco, Damianos S. Skopelitis, and Marja C.P. Timmermans

13. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs

Andrea Tovar-Aguilar, Karla A. S?nchez-Elizondo, Alejandra Rodr?guez-Rodr?guez, Marcos I. Gonz?lez-Jaime, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Jesus A. Badillo-Corona, and No? Dur?n-Figueroa

14. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy

Andrea G?mez-Felipe and Stefan de Folter

15. Degradome Sequencing in Plants

Shih-Shun Lin, Yihua Chen, and Mei-Yeh Jade Lu

16. Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System

Damien Formey, Jos? ?ngel Mart?n-Rodr?guez, and Georgina Hern?ndez

17. Th

• Format: Hardcover
• Language: English
• ISBN: 1493990411
• ISBN13: 9781493990412
• Release Date: January 2019
• Publisher: Humana
• Length: 363 Pages
• Weight: 1.91 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"