G Protein-Coupled Receptor Signaling: Methods and Protocols

Part I: Probing Structural Properties of GPCRs

1. Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics

Chenyi Liao, Victor May, and Jianing Li

2. Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs

Katsufumi Tomobe, Eiji Yamamoto, and Kenji Yasuoka

3. Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies

Fabian Bumbak, Ross A.D. Bathgate, Daniel J. Scott, and Paul R. Gooley

4. A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties

Anne Zemella, Theresa Richter, Lena Thoring, and Stefan Kubick

Part II: Tweaking Ligands for GPCR Studies

5. Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions

Marleen Van Troys, Willem Vannecke, Christophe Ampe, and Annemieke Madder

6. Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands

Amanda Acosta-Ruiz, Johannes Broichhagen, and Joshua Levitz

7. Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs

Srid?vi M. Ramanoudjame, Lucie Esteoulle, St?phanie Rich?, Jean-Fran?ois Margathe, Thierry Durroux, Iuliia A. Karpenko, and Dominique Bonnet

Part III: Bioluminescence and Fluorescence Resonance Transfer Energy Approaches

8. Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology

Joyce Heuninck, Candide Hounsou, Elodie Dupuis, Eric Trinquet, Bernard Mouillac, Jean-Philippe Pin, Dominique Bonnet, and Thierry Durroux

9. Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches

Kyla Bourque, Dominic Devost, Asuka Inoue, and Terence E. H?bert

10. Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays

James H. Felce, John R. James, and Simon J. Davis

11. Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions

Amina M. Bagher, Melanie E.M. Kelly, and Eileen M. Denovan-Wright

12. Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters

Andreas Koschinski and Manuela Zaccolo

Part IV: Exploring GPCR Signaling Properties

13. Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays

Genevi?ve Hamel-C?t?, Fanny Lapointe, and Jana Stankova

• Format: Hardcover
• Language: English
• ISBN: 1493991205
• ISBN13: 9781493991204
• Release Date: April 2019
• Publisher: Humana
• Length: 406 Pages
• Weight: 2.09 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"